Remove the cassette from the foil packaging and lay the cassette flat. Use a pipette to obtain a 50 microliter sample of serum. Pipette the sample onto the middle circle on the cassette, then wait 60 seconds. Push down the tab on the cassette until you hear a click. Add two drops of buffer to the other circle on the cassette. Wait 20 minutes and then read the test.
To read the test open your phone to our application. Then insert the cassette through the slot on the reader with the window of the cassette facing the phone. Turn on the lightbox and insert the phone with the reader into the lightbox. Click “read test” in the application. The assay will be performed and the result will be provided with date and time stamp.